Fig. 2

O-PMs increased fibronectin expression in A549 cells. a A549 cells were treated with 0, 25, 50, or 100 μg/mL O-PMs for 24 h. Fibronectin expression in the cell lysates was examined by Western blot. b A549 cells were treated with or without 5 mM NAC for 1 h, and were then treated with or without 100 μg/ml of O-PMs for 24 h. Fibronectin expression was evaluated by Western blot. c The detection and distribution of fibronectin expression in A549 cells with or without 100 μg/mL O-PMs for 24 h was determined by immunocytochemistry. Bar = 100 μm. d The effect of NAC on the migratory abilities of O-PMs-treated A549 cells was determined by a wound healing assay. A representative image of each group at different time points after wound formation is shown. The red lines represent the wound boundaries. The percentage of the wound area is expressed as the mean ± SEM, n = 3. Bar = 100 μm. e A549 cells were pre-treated with 5 mM NAC and incubated for 1 h. Cells treated with or without NAC were treated with 100 μg/mL O-PMs for 24 h. The expression levels of E-cadherin and vimentin were measured by Western blot. *p < 0.05 compared to control (Con) cells; †p < 0.05 compared to O-PMs-treated cells