Fig. 5
O-PMs-induced EMT is closely related to the fibronectin receptor. a A549 cells were pretreated with 50 μM or 100 μM ATN-161 for 30 min, and then treated with 100 μg/mL O-PMs for 24 h. The expression levels of fibronectin, ETS-1, E-cadherin, and vimentin were examined by Western blot. b The effect of ATN-161 on the phosphorylation of NF-κB p65 was determined by Western blot. A549 cells were pretreated with 100 μM ATN-161 for 30 min and then treated with 100 μg/mL O-PMs for 15 and 30 min. c The effect of ATN-161 on the migratory abilities of O-PMs-treated A549 cells was determined by a wound healing assay. Representative images of each group at the indicated time points after wound formation are shown. The red lines represent the wound boundaries. The percentage of the wound area is expressed as the mean ± SEM, n = 3. Bar = 100 μm. *p < 0.05 vs. control (Con) cells; †p < 0.05 vs. O-PMs-treated cells