Fig. 6

PMs induced collagen deposition and EMT-related protein expression in lung tissues in vivo. WT mice were treated with 200–350 μg/mouse PMs by intratracheal instillation. After 7 or 14 days, the mice were euthanized and lung tissue was collected. a Collagen deposition in lung tissue was determined by Masson’s trichrome staining. Blue: collagen. Bar = 100 μm. b The expression of ETS-1, fibronectin, E-cadherin and vimentin in mouse lung tissue was measured by immunohistochemical staining. The dotted rectangle in the left image is enlarged and displayed in the right image. c Western blot was used to measure the expression levels of ETS-1, fibronectin, E-cadherin and vimentin in mouse lung tissue. Bar = 100 μm. *p < 0.05 vs control (Con) group