Fig. 3

The selection of an optimal wavelength to quantify CNMs without interference from tissue homogenates. a, Absorbance of CNMs or vehicle control [VEH (DW), DW with 3% FBS]. b, Absorbance of vehicle control [VEH (tissue), tissue lysates in DW] and CNMs collected from a mixture of 25 μg/mL CNMs and 0.02 g (dry weight) lung tissue homogenates following treatment with 200 μg of proteinase K (PK). The insert figure represents that the absorbance of the bio-components was lower at 750 nm after treatment with PK, making it the optimal wavelength to quantify CNMs without interference from the tissue homogenate