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Table 2 The accuracy (%) of the UV-Vis spectrophotometer technique when measuring CNM concentration

From: Carbon nanomaterial-derived lung burden analysis using UV-Vis spectrophotometry and proteinase K digestion

CNMs

Target concentration (μg/mL)

10

20

30

300a

CB

 Measured

9.53 ± 0.28

19.37 ± 0.66

28.76 ± 0.47

285.49 ± 1.04

 Accuracy %

95.3 ± 2.84

96.8 ± 3.33

95.8 ± 1.57

95.1 ± 0.34

ND

 Measured

9.51 ± 0.26

19.07 ± 0.76

28.52 ± 1.03

286.06 ± 4.05

 Accuracy %

95.1 ± 2.61

95.3 ± 3.82

95.0 ± 3.43

95.3 ± 1.35

MWCNT

 Measured

9.69 ± 0.18

19.29 ± 0.57

28.75 ± 1.11

285.12 ± 4.55

 Accuracy %

96.9 ± 1.84

96.4 ± 2.85

95.8 ± 3.70

95.0 ± 1.51

CNF

 Measured

9.42 ± 0.56

19.03 ± 0.57

28.69 ± 1.06

286.04 ± 2.22

 Accuracy %

94.2 ± 5.67

95.1 ± 2.88

95.6 ± 3.55

95.3 ± 0.74

GNP

 Measured

9.63 ± 0.11

19.48 ± 0.87

29.22 ± 1.10

289.88 ± 9.92

 Accuracy %

96.2 ± 1.12

97.4 ± 4.37

97.4 ± 3.68

96.6 ± 3.30

  1. The accuracy (%) was calculated by comparison with the target concentration weighed during sample preparation
  2. aThe target concentrations were selected as 10, 20, and 30 μg/mL for non-diluted samples and 300 μg/mL for samples needed dilution
  3. The data are presented as mean ± SEM from four independent measurements
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Particle and Fibre Toxicology

ISSN: 1743-8977

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