Occupational exposure to carbon black nanoparticles increases inflammatory vascular disease risk: an implication of an ex vivo biosensor assay

Table 2 mRNA expression of seven genes in primary hCAECs treated with sera from 106 non-CBPs and 82 CBPs^a

Gene	Non-CBP	CBP	Estimate (95%CI)	RQ (95%CI)^b	P
Biosensor PC1	0.62 (− 0.27–1.60)	− 0.26 (− 0.92–0.17)	− 0.92 (− 1.31 – − 0.53)	1.89 (1.44–2.48)	< 0.0001
CCL2	−7.10 (−8.05 – − 6.35)	−7.67 (− 8.47 – − 7.15)	− 0.57 (− 1.00 – − 0.13)	1.48 (1.09–2.00)	0.011
CCL5	− 0.76 (− 1.27 – − 0.28)	−1.29 (− 1.94 – − 0.74)	− 0.48 (− 0.77 – − 0.19)	1.39 (1.14–1.71)	0.0012
CXCL8	−7.05 (− 9.33 – −5.10)	− 10.29 (− 12.11 – − 8.26)	−3.18 (− 4.01 – − 2.35)	9.06 (5.10–16.11)	< 0.0001
CXCL12	− 2.63 (− 3.30 – − 2.21)	−2.33 (− 2.65 – − 1.99)	0.62 (0.23–1.01)	0.65 (0.50–0.85)	0.0018
ICAM	−4.56 (− 5.96 – − 3.83)	−4.96 (− 5.77 – − 4.42)	− 0.39 (− 0.72 – − 0.06)	1.31 (1.04–1.65)	0.021
SELP	0.07 (− 0.32–0.46)	− 0.02 (− 0.32–0.37)	− 0.15 (− 0.47–0.18)	1.11 (0.88–1.39)	0.37
VCAM	−1.71 (− 2.66 – − 0.94)	−2.59 (− 3.15 – − 1.84)	− 0.69 (− 1.14 – − 0.24)	1.61 (1.18–2.20)	0.0027

CBP Carbon black packer, RQ Relative quantification, CI Confidence interval, hCAEC Human coronary artery endothelial cell, GLM Generalized linear model
^aBiosensor PC1was the major principal component extracted from delta Cts of the seven genes. Delta Cts of target genes relative to endogenous control gene (TBP) were expressed as median and interquartile range. Estimates were calculated based on biosensor PC1 or delta Ct of individual genes using GLM with adjustment for age, overweight and obesity, current smoking status, packyears, and passage of cells
^bRelative quantification was calculated as RQ = 2^-(estimate)

ISSN: 1743-8977