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Fig. 2 | Particle and Fibre Toxicology

Fig. 2

From: Zinc oxide nanoparticles effectively regulate autophagic cell death by activating autophagosome formation and interfering with their maturation

Fig. 2

The role of ROS in ZnO NPs-induced cytotoxicity. a PI staining to show the cytotoxicity of ZnO NPs in PC12 cells. These cells were exposed to 15 μg/mL ZnO NPs for the indicated times, and the percentage of dead cells was measured by PI staining. Flow cytometry results were displayed in histogram plot. b ROS levels after ZnO NPs exposure were detected using the ROS indicator DCF. Effect of NAC on ZnO NPs-induced ROS level by DCFH-DA staining (c) and cytotoxicity by PI staining (d). Cells were pretreated with 2 mM NAC for 1 h, followed by exposure to 15 μg/mL ZnO NPs for 6 h or 12 h, respectively. MFI: mean fluorescence intensity. Data were representative of at least three independent experiments

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