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Fig. 5 | Particle and Fibre Toxicology

Fig. 5

From: Zinc oxide nanoparticles effectively regulate autophagic cell death by activating autophagosome formation and interfering with their maturation

Fig. 5

JNK activation contributes to ZnO NPs-induced PC12 cell death. Effects of ZnO NPs on p38, ERK (a), and JNK (b) signaling pathways. Activation status of the p38, ERK, and JNK was analyzed in whole cell proteins by immunoblotting with antibodies to phosphorylated p38, ERK, JNK and total p38, ERK, JNK, respectively. The densitometry of those bolts from at least three independent experiments was shown in Figure S8C and D. Effect of JNK, p38 and ERK inhibitors on ZnO NPs-induced cell death as shown by PI staining (c) and CCK8 assay (d). Cells were pretreated with 10 μM JNK inhibitor SP600125, 3 μM p38 inhibitor SB203580 and 10 μM ERK inhibitor PD98059 for 1 h, followed by exposure to 15 μg/mL ZnO NPs for 12 h. Data from at least three independent experiments were expressed as the means ± SD. ***p < 0.001 compared with the untreated control

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