Fig. 2
Short term exposure to DEP reduces epithelial barrier function without causing cytotoxicity. a TEER measurements of cells exposed to indicated concentrations of DEP at time 0, 6, and 8.5 h after application of DEP. One-Way ANOVA with Tukey’s HSD, ****P < 0.0001 Vehicle vs 25 μg/cm2 DEP, #P < 0.05, ####P < 0.0001 Vehicle vs 50 μg/cm2 DEP, &P < 0.05, &&&& < 0.0001 25 μg/cm2 vs 50 μg/cm2 DEP. Data from three independent experiments, N = 5–6 replicates per treatment per time point per experiment. b 4 kDa FITC-Dextran permeability measured at 8.5 h after application of DEP. One-Way ANOVA with Tukey’s HSD, ****P < 0.0001. Data from three independent experiments, N = 5–6 replicates per treatment per experiment. c Fold change LDH release normalized to average Vehicle LDH release. One-Way ANOVA, *P < 0.05. Data from three independent experiments, N = 5–6 replicates per treatment per time point per experiment