Fig. 4

siRNA mediated knockdown of Tricellulin recapitulates barrier dysfunction caused by DEP exposure. a Tricellulin siRNA significantly reduces Tricellulin protein 3 days post transfection. One-Way ANOVA with Tukey’s HSD, **P < 0.01. Representative Western blot from one experiment, densitometry results from two independent experiments, N = 4 replicates per treatment per experiment. b Tricellulin knockdown causes a reduction in TEER. Student’s t-test with Holm-Sidak method, *P < 0.05, ****P < 0.0001. Data from three independent experiments, N = 4 replicates per experiment per time point. c Tricellulin knockdown cells exhibit increased permeability to 4 kDa FITC-Dextran 3 days post transfection. Student’s t-test, ****P < 0.0001. Data from three independent experiments, N = 4 replicates per treatment per experiment