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Fig. 3 | Particle and Fibre Toxicology

Fig. 3

From: Exposure to diesel exhaust particles results in altered lung microbial profiles, associated with increased reactive oxygen species/reactive nitrogen species and inflammation, in C57Bl/6 wildtype mice on a high-fat diet

Fig. 3

Exposure to diesel exhaust particles results in increased expression of TNF-α. Representative images of tumor necrosis factor (TNF)-α expression in the lungs of C57Bl/6 mice on a control (LF; a-c) or high-fat (HF; g-i) diet exposed to saline (control) or on a LF (d-f) or HF diet (j-l) exposed to diesel exhaust particles (DEP – 35 μg PM) twice a week for a total of 30 days. Red fluorescence indicates TNF-α expression, blue fluorescence is nuclear staining (Hoechst). Right panels (c, f, i, l) are merged figures of left (blue; a, d, g, j) and center (red; b, e, h, k) panels. m Graph of histology analysis of lung TNF-α fluorescence and n mean normalized gene expression of TNF-α mRNA transcript expression within the lungs, as determined by RT-qPCR. 40x magnification; scale bar = 100 μm. *p < 0.05 compared to LF Control, †p < 0.05 compared to HF Control, ‡p < 0.05 compared to LF DEP by two way ANOVA

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Particle and Fibre Toxicology

ISSN: 1743-8977

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