Fig. 4

Mitochondrial response following exposure to wood tar extracts. Cells were exposed to water-soluble extracts from wood tar solution (at a concentration of 0.2 mg/ml or 2 mg/ml) for one or 5 h prior to analysis, as specified. Determination of the mitochondrial OCR (a measure of mitochondrial respiration) and ECAR (a measure of glycolysis) was performed with a Seahorse analyzer. a Description of the mitochondrial respiration (mitochondrial stress) and b Seahorse glycolysis assays. Selected results showing c the basal and mean OCR and d the basal and mean ECAR following injection of inhibitors and substances after 5 h of exposure are shown. e The OCRs after 1 h of exposure and f the ECARs after 1 h of exposure are shown. g MtDNAcn. The data represent the mean ± SD. These experiments were performed in triplicate and repeated twice