Fig. 7
From: Differential effects of intense exercise and pollution on the airways in a murine model
Airway permeability and integrity. a Surfactant protein D (SP-D) concentrations in serum were measured using ELISA (R&D Systems). b Uric acid concentrations in serum were measured using ELISA (ThermoFisher). c Protein levels in bronchoalveolar lavage fluid were measured using a Bradford assay (Biorad). d OCLN mRNA expression, e ZO-1 mRNA expression, f Cldn-1 mRNA expression, g Cldn-3 mRNA expression, h Cldn-4 mRNA expression and i Cldn-18 mRNA expression in lung tissue were measured using RT-qPCR. Data shown as mean with SD (n = 9 mice per group). *p < 0.05, **p < 0.01, ***p < 0.001 comparing individual groups. & = DEP-effect, # = Exercise-effect, + = Interaction-effect (Two-Way ANOVA)