TY - JOUR AU - Lison, Dominique AU - Ibouraadaten, Saloua AU - van den Brule, Sybille AU - Todea, Milica AU - Vulpoi, Adriana AU - Turcu, Flaviu AU - Ziemann, Christina AU - Creutzenberg, Otto AU - Bonner, James C. AU - Ameloot, Marcel AU - Bové, Hannelore PY - 2021 DA - 2021/02/18 TI - Femtosecond pulsed laser microscopy: a new tool to assess the in vitro delivered dose of carbon nanotubes in cell culture experiments JO - Particle and Fibre Toxicology SP - 9 VL - 18 IS - 1 AB - In vitro models are widely used in nanotoxicology. In these assays, a careful documentation of the fraction of nanomaterials that reaches the cells, i.e. the in vitro delivered dose, is a critical element for the interpretation of the data. The in vitro delivered dose can be measured by quantifying the amount of material in contact with the cells, or can be estimated by applying particokinetic models. For carbon nanotubes (CNTs), the determination of the in vitro delivered dose is not evident because their quantification in biological matrices is difficult, and particokinetic models are not adapted to high aspect ratio materials. Here, we applied a rapid and direct approach, based on femtosecond pulsed laser microscopy (FPLM), to assess the in vitro delivered dose of multi-walled CNTs (MWCNTs). SN - 1743-8977 UR - https://doi.org/10.1186/s12989-021-00402-5 DO - 10.1186/s12989-021-00402-5 ID - Lison2021 ER -