Fig. 6

Macrophage responses to the first (A, B) and second set of MWCNTs (C, D) tentatively adjusted to the in vitro delivered dose as determined by FPLM. For cytotoxicity data (panels A and C), the relative (remaining) WST1 activities recorded at 9 μg/ml in THP-1 cells (Figs. 4a and 5s, n = 4) was transformed to reflect the net cytotoxic activity (i.e., 1 - relative WST-1 activity). For each CNT sample, the mean of these values was calculated. This mean was then divided by the mean FPLM signal recorded in THP-1 cells after exposure to the same applied dose (9 μg/ml) (Figs. 4e and 5e, n = 3). The same procedure was applied for IL-1β (panels B and D), i.e., for each CNT sample, the mean of the IL-1β concentrations recorded at 9 μg/ml was calculated (Figs. 4b and 5b, n = 4) and then divided by the mean FPLM signal recorded in THP-1 cells after exposure to 9 μg/ml (Figs. 4e and 5e, n = 3)