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Fig. 3 | Particle and Fibre Toxicology

Fig. 3

From: Effects of metal nanoparticles on tight junction-associated proteins via HIF-1α/miR-29b/MMPs pathway in human epidermal keratinocytes

Fig. 3

Dose- and time-dependent induction of MMP-2 and MMP-9 activities in HaCaT cells exposed to Nano-Ni. HaCaT cells were exposed to 10 or 20 μg/mL of Nano-Ni or Nano-TiO2 for 24 h (a & b), or to 20 μg/mL of Nano-Ni for 12, 24, 48, or 72 h (c & d). Cells without treatment were used as control. MMP-2 and MMP-9 activities were determined in the conditioned media by gelatin zymography assay. A and C are the results of a single experiment, while B and D are normalized band densitometry readings averaged from three independent experiments ± SE. Supernatants collected from serum-free cultured HT1080 cells were used as a molecular weight marker for MMP-2 and MMP-9. * Significant difference as compared with the control group, p < 0.05; # Significant difference as compared with the same dose of Nano-TiO2-treated group, p < 0.05

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