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Fig. 6 | Particle and Fibre Toxicology

Fig. 6

From: Effects of metal nanoparticles on tight junction-associated proteins via HIF-1α/miR-29b/MMPs pathway in human epidermal keratinocytes

Fig. 6

HIF-1α nuclear accumulation in HaCaT cells exposed to Nano-Ni. Expression of HIF-1α in HaCaT cells was determined by Western blot after cells were exposed to 10 or 20 μg/mL of Nano-Ni or Nano-TiO2 for 24 h (a), or to 20 μg/mL of Nano-Ni for 12, 24, 48, or 72 h (c). The representative results of three Western blot experiments are shown. Cells without treatment were used as control. Equal protein loading was verified by Coomassie Brilliant Blue staining. b & d are normalized band densitometry readings averaged from three independent experiments ± SE. * Significant difference as compared with the control group, p < 0.05

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