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Fig. 12 | Particle and Fibre Toxicology

Fig. 12

From: Respiratory and systemic impacts following MWCNT inhalation in B6C3F1/N mice

Fig. 12

Changes in monocyte subpopulations and neutrophil populations in the spleen following 30-day MWCNT exposure + 10 days recovery to MWCNT. At multiple doses of particle exposure, splenic cell populations were assessed for changes using flow cytometry. Spleens were removed and disrupted to achieve single-cell suspensions for analysis. a Change in total monocyte populations (CD11b+ Ly6Clo/hi), where a nonsignificant increase in the total monocyte population were observed. b Shows changes in the inflammatory monocyte populations with significant increases in iMono/M-MDSC (CD11b+ Ly6Chi) for the highest 2 doses compared the low dose and the air control *** P < 0.001. c Shows changes in the resident monocyte populations with nonsignificant and significant decreases in rMono (CD11b+ Ly6Clo) for all particle doses compared to the air control *** P < 0.001. d Neutrophil (PMN, CD11b+ Ly6G+) percent showed no significant changes across dose, but there was a nonsignificant increase in the 0.2 mg/m3 dose. Mono = total monocytes; iMono = inflammatory monocytes; M-MDSC = monocytic myeloid-derived suppressor cells; rMono = resident monocytes, and PMN = neutrophils. All data expressed as mean ± SEM (n = 8)

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