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Fig. 2 | Particle and Fibre Toxicology

Fig. 2

From: Airborne particulate matters induce thrombopoiesis from megakaryocytes through regulating mitochondrial oxidative phosphorylation

Fig. 2

QFF-PM2.5 exposure promoted thrombopoiesis in megakaryocytes. a The fluorescence images of thrombopoiesis in megakaryocytes are characterized by immunostaining with wheat germ agglutinin (WGA, red fluorescence). The green arrows denote the budding cells. Scale bar = 15 μm. b Quantitative analysis of thrombopoiesis by counting the budding cell numbers in random views (n = 4). *p < 0.05, or **p < 0.01 versus the control. The blank filter extract (i.e. QFF-Ctr) and 1 ng/mL TPO were used as QFF-PM2.5 free and positive controls, respectively. c, d The representative TEM images of megakaryocytes. The yellow arrows refer to the features of mature megakaryocytes and thrombopoiesis, such as sponge-like edges, indicating the platelet demarcation membrane system. The red arrows indicate the fine particles in megakaryocytes. Scale bar = 2 μm. The exposure concentration of QFF-PM2.5 was 100 ng/mL, and the exposure lasted for 12 d

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