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Fig. 4 | Particle and Fibre Toxicology

Fig. 4

From: Size and surface modification of silica nanoparticles affect the severity of lung toxicity by modulating endosomal ROS generation in macrophages

Fig. 4

Size and surface modification indicate uptake and subsequent inflammatory response of Raw264.7 cells to silica-NPs. A. Cellular uptake of silica particles was studied by immunofluorescence in vitro. Raw264.7 cells, a murine phagocytic cell line, were exposed to different silica-NPs, and cellular uptake was assessed 6 h later by confocal microscopy. In the images, blue indicates cell nucleus, green indicates FITC-labeled silica particles, and red indicates Alexa 594-labeled anti-F4/80 antibody as a cell surface marker of murine macrophages. Scale bars in low magnification (top panels) = 50 μm, and in high magnification (bottom panels) = 10 μm. B-C. Flow cytometric detection of intracellular silica-NP accumulation in Raw264.7 cells after 6 h endocytosis. Representative scatter plots showing endocytosis of FITC-labeled silica-NPs are shown in B. The graph in C shows the proportions of cells that endocytosed FITC-labeled silica particles. D-F. Cytokine expressions in RAW264.7 cells 6 h after exposure to silica-NPs. Gene expressions of inflammatory cytokine genes (MIP1α(D), MIP2(E), and TNF-α(F)) were determined by qRT-PCR. Results are shown as mean ± SEM of five independent experiments

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