Fig. 3
Workflow and DEPs in lung tissue proteomics analysis. a Experimental workflow for lung tissue proteomics analysis. The lung tissues from five rats in individual groups were lysed, digested, and pooled to generate six pooled peptide samples. Each pooled sample was labelled with one of the TMTs separately. The labelled peptides were then combined for reverse phase StageTip fractionation, followed by LC-MS/MS analysis and database searching for protein identification and quantitation. A total of 2673 proteins were identified, of which 2526 proteins were quantified. b The overlapping of DEPs in the 3 M-GAS and 3 M-PM1 groups, progressive GAS and PM1 exposures, and particle-specific DEPs under 3- and 6-month exposure