Fig. 6From: Prolonged exposure to traffic-related particulate matter and gaseous pollutants implicate distinct molecular mechanisms of lung injury in ratsFunctional validation of selected proteins, oxidative stress, and IL-6 in lung tissues. a, b, and c present the statistical results of Western blot analysis for C3, Chp1, and Serpina3 proteins in lung tissues, respectively. Vinculin was used as the loading control. The expression level of each protein was further normalized to that in the reference sample running in every gel for between-gel comparison. d and e present the ELISA results of 8-isoprostane and IL-6, representing the status of oxidative stress and inflammation in lung tissues, respectively. The quantities of 8-isoprostane and IL-6 were normalized to the total protein lysate before comparison. * p < 0.05Back to article page