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Fig. 6 | Particle and Fibre Toxicology

Fig. 6

From: Silica-coated magnetic nanoparticles activate microglia and induce neurotoxic d-serine secretion

Fig. 6

Microglial activation and D-serine distribution in MNPs@SiO2(RITC)-treated mouse brains.a Schematic representation of the in vivo analysis. b Immunohistochemical analysis of the cerebellum regions of mice. Low-magnification images are merged with the florescence images of Hoechst 33,342 (blue), MNPs@SiO2(RITC) (red), Iba1 (green), and D-serine (violet) to show region-specific structures and the distribution of MNPs@SiO2(RITC) and D-serine. Scale bar = 100 μm. Magnified images are separated into Hoechst 33,342, MNPs@SiO2(RITC), Iba1, and D-serine and Iba1-based 3D-rendered images. White scale bar = 10 μm. c Filament length determined from the 3D-rendered images. d Quantification of nuclei in brain sections. *p < 0.05 vs. control

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