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Fig. 5 | Particle and Fibre Toxicology

Fig. 5

From: The mechanism of cell death induced by silver nanoparticles is distinct from silver cations

Fig. 5

Reduction of hydrogen peroxide through PEG-catalase attenuates Ag+ but not AgNP mediated cell death. A SUM159 or B iMEC cells were exposed to increasing doses of AgNPs with or without 100 units/mL of PEG-catalase. Cell viability following 24 h AgNP exposure was quantified by MTT assay. C IC50 was calculated using GraphPad Prism. Data used to calculate IC50’s were obtained from 6 technical replicates per dose and 3 independent experiments (biological replicates). D SUM159 or E iMEC cells were exposed to increasing doses of Ag+ with or without 100 units/mL of PEG-catalase. Cell viability following 24 h Ag+ exposure was quantified by MTT assay. F IC50 was calculated using GraphPad Prism. Data used to calculate IC50’s were obtained from 6 technical replicates per dose and 3 independent experiments (biological replicates). Statistical analysis in C and F was performed by students T-Test. Statistical differences are indicated (**p < 0.01; ****p < 0.0001). G, H Long term proliferative potential was assessed via clonogenic assay following 24 h AgNP exposure in the presence of 100 units/mL of PEG-catalase in G SUM159 and H iMEC cells. I, J Long-term proliferative potential was assessed via clonogenic assay following 24 h Ag+ exposure in the presence of 100 units/mL of peg-catalase in I SUM159 and J iMEC cells. Data in G–J are presented as relative surviving fraction based upon clonogenic growth normalized to plating efficiency. Statistical analysis was performed by one-way ANOVA followed by post-hoc Tukey Test. Statistical differences are indicated (*p < 0.05)

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