Nanomaterial- and shape-dependency of TLR2 and TLR4 mediated signaling following pulmonary exposure to carbonaceous nanomaterials in mice

Table 2 BAL cell counts in mice 24 h post exposure

	Control	LPS	CB	CNT	GO
	0 µg	4 µg	162 µg	54 µg	18 µg
WT
Total BAL cells (× 10³)	73.1 ± 34.9	824 ± 404***	191 ± 61.2**	151 ± 33.6**	293 ± 85.9***
Macrophages (× 10³)	59.9 ± 19.3	76.8 ± 47.3	23.9 ± 15.4**	50.8 ± 8.74	52.4 ± 13.9
Lymphocytes (× 10³)	0.51 ± 0.45	6.14 ± 6.96	1.30 ± 1.35	2.20 ± 1.51	1.61 ± 1.10
Neutrophils (× 10³)	4.75 ± 4.93	724 ± 338**	133 ± 45.4***	70.8 ± 31.9**	218 ± 73.9**
Eosinophils (× 10³)	8.30 ± 22.8	1.37 ± 3.06	28.2 ± 23.4**	21.3 ± 15.0*	17.1 ± 9.82*
Epithelial (× 10³)	4.40 ± 3.60	15.6 ± 14.7*	5.52 ± 3.95	5.61 ± 1.94	4.13 ± 2.65
Tlr2 KO
Total BAL cells (× 10³)	73.1 ± 34.9	475 ± 106***	184 ± 31.0**	133 ± 47.7*	239 ± 33.2**
Macrophages (× 10³)	59.9 ± 19.3	30.1 ± 17.9*	27.1 ± 17.3*	35.9 ± 18.6	81.4 ± 22.9
Lymphocytes (× 10³)	0.51 ± 0.45	1.27 ± 1.47	4.19 ± 2.32**^{, a}	2.65 ± 2.93*	6.46 ± 7.70
Neutrophils (× 10³)	4.75 ± 4.93	405 ± 66.0**	140 ± 31.9**	60.0 ± 13.5**	140 ± 44.1**
Eosinophils (× 10³)	8.30 ± 22.8	0.00 ± 0.00	9.65 ± 7.75*	27.0 ± 37.5*	8.45 ± 10.8*
Epithelial (× 10³)	4.40 ± 3.60	3.29 ± 3.23	3.13 ± 1.22	7.34 ± 3.93	6.05 ± 1.27

All values are presented as mean ± SD. There are no statistical significant difference between the vehicle controls, therefore these are pooled in the statistical analyses (WT + Tlr2 KO). A symbol (*) denotes P ≤ 0.05, (**) P ≤ 0.01, (***) P ≤ 0.001 compared to vehicle control. Comparing Tlr2 KO and WT, a letter (^a) denotes P ≤ 0.05, (^b) P ≤ 0.01

ISSN: 1743-8977