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Fig. 2 | Particle and Fibre Toxicology

Fig. 2

From: Silica-coated magnetic-nanoparticle-induced cytotoxicity is reduced in microglia by glutathione and citrate identified using integrated omics

Fig. 2

Transcriptomic and proteomic changes in 0.1 µg/µl MNPs@SiO2(RITC)-treated BV2 cells. a Heat map of 121 genes with altered expression in the RNA-seq analysis. b Gene Ontology analysis of the transcriptome for MNPs@SiO2(RITC)-treated cells. c Analysis of the transcriptomic network with prediction using IPA in MNPs@SiO2(RITC)-treated BV2 cells. The analysis involved a fold change cut-off value ± 1.5. Red and green nodes indicate genes that were up-regulated and down-regulated, respectively, compared to the control. Orange and blue arrows indicate prediction of activation and inhibition, respectively. Details for shape and color, which are originated from Ingenuity Systems (http://www.ingenuity.com), are provided in Figs. S9 and S11. d qPCR analysis was performed to determine gene expression in each group, with GAPDH as an internal control. Data represent means ± standard deviation of three independent experiments. *p < 0.05 versus control and #p < 0.05 versus 0.01 µg/µl MNPs@SiO2(RITC)-treated cells. e Heat map of 45 proteins with altered expression in LC–MS/MS analysis. f Gene Ontology analysis of the proteome for MNPs@SiO2(RITC)-treated cells. g Functional analysis of the proteomic network with prediction using IPA in MNPs@SiO2(RITC)–treated cells. The analysis used a fold-change cut-off value ≥  ± 1.5. Details for shape and color are provided in Figs. S9 and S11. h The relative abundance levels of Fas, Gstm1, Gstm5, Icam1, Nfkb1, and Src according to LC–MS/MS analysis. Data represent means ± standard deviation of the two TMT ratios. *p < 0.05 versus control

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