Fig. 6
Evaluation of MNPs@SiO2(RITC)-induced microglial activation and effects of GSH and citrate in vivo. a Schematic of the in vivo experiment. b Immunohistochemical analysis of the hippocampal regions of the mouse brain. Low-magnification images are merged with florescence of Hoechst 33,342 (blue), MNPs@SiO2(RITC) (red), and Iba1 (green) to show region-specific structure and distribution of MNPs@SiO2(RITC). Black scale bar = 100 μm. Magnified images are separated into Hoechst 33,342 (blue), MNPs@SiO2(RITC) (red), and Iba1 (green), and Iba1-based 3D rendering images. White scale bar = 10 µm. c Determined filament length from 3D rendering images of the hippocampus. d Representative immunoblotting data related to microglia activation. β-Actin served as an internal control. Normalized expression of Iba1 e, CD40 f, and CD11b g in hippocampal tissue lysates. h Immunohistochemical analysis of the thalamus in the mouse brain. i Determined length of a filament from 3D rendering images of thalamic regions. j Representative immunoblotting data related to microglia activation. β-Actin was used as an internal control. Normalized expression of Iba1 k, CD40 l, and CD11b m in lysates of the thalamus. Data represent means ± standard error of three independent experiments. *p < 0.05 versus control, #p < 0.05 versus MNPs@SiO2(RITC)-treated mice according to one-way ANOVA