Fig. 3

Single-cell landscape in murine lungs exposed to coal dust. A Schematic of the experimental workflow used to define the pulmonary cells of 9-month-exposed mice. B Quality control of unique cell molecular identifiers (UMI) and mitochondria. C, D tSNE plot and clustering of 42,252 cells from coal-exposed mice (n = 2) and vehicle control mice (n = 2), classified into 19 clusters and seven distinct cellular types. E Violin plot showed the feature of vital lineage-associated in the mouse lung clusters. F Average proportion of eight main types of lung cells in coal dust-exposed lungs and control lungs