Fig. 6

After long-term coal dust exposure, the heterogeneity, phenotypic diversity, and functional alternation of pulmonary macrophages. A tSNE plot view of 3582 macrophages, color-coded by the associated cluster (top) or the assigned cell type (bottom). B The fraction of macrophage subclusters from each of the four mice, box plots of the number of cells (with the plot center corresponding to the median). C The relative increase or decrease of the macrophages of clusters in the coal-exposed lungs was observed, compared with the control (positive represents more, negative represents less); D Single-Cell RNA-SEQ identified seven macrophage populations in mouse lungs. The tSNE diagram shows an aggregation of 3582 cells from 4 mice. E The volcano plot of the SDEGs of pulmonary macrophages from the coal-exposed mice and the control. A total of 129 upregulated genes are presented in red, while 266 downregulated genes are presented in blue (bar chart). F Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway classification map. The genes were divided into six branches according to the biological pathways. G The top 20 significantly pathways are enriched by KEGG based on significantly differentially expressed genes (SDEGs). H The Gene Ontology (GO) enrichment analysis shows the association between the genes and phenotypes of coal dust-exposed mice