Fig. 5
Expression of CYP1A1 and CYP1B1 after combined exposure to DEPB7 and α-quartz. HBEC3-KT cells were exposed to 25 μg/mL (2.6 μg/cm2) DEPB7 and 50–100 μg/mL (5.3–10.5 μg/cm2) α-quartz for 2 h (i), 6 h (ii) and 12 h (iii), alone or in combination. The expression of CYP1A1 (A) and CYP1B1 (B) was determined using qPCR. Results are expressed as fold change compared to unexposed cells and presented as boxplots of 4 independent experiments (Box: 25th–75th percentile, whiskers: minimum and maximum values, line: median, +: mean). Statistically significant differences were determined using two-way repeated measures ANOVA followed by Dunnett’s and Å Ãdák's post-tests and were calculated based on normalized CT values. *Statistically significant difference from the respective control at 0 μg/mL α-quartz. #Statistically significant difference between exposure groups