Fig. 7
Cytokine responses after exposure to α-quartz in combination with DEPB0 or organic extracts of DEPB0. HBEC3-KT cells were exposed for 24 h to 25 μg/mL (2.6 μg/cm2) of DEPB0 and corresponding concentrations of DEPB0 extracts (DEE), alone or in combination with 100 μg/mL (10.5 μg/cm2) α-quartz. The release of CXCL8 (A) and IL-1β (B) in the cell culture supernatants was measured using ELISA. Results are presented as boxplots of 5 independent experiments (Box: 25th–75th percentile, whiskers: minimum and maximum values, line: median, +: mean). Statistically significant differences were determined using two-way repeated measures ANOVA followed by Dunnett’s and Å Ãdák's post-tests. All values were log-transformed prior to statistical analysis to satisfy model assumptions. *Statistically significant difference from the respective control. #Statistically significant difference between exposure groups