Skip to main content

Table 2 Internal dose of disesel exhaust exposure and biosensor gene expressions in primary HCAECs treated with sera from 133 DETs and 126 non-DETs

From: Circulatory metabolites trigger ex vivo arterial endothelial cell dysfunction in population chronically exposed to diesel exhaust

Variable

Internal exposure category of disesel exhaust

Trend testa

Low

Medium

High

Estimate (95%CI)

P value

# non-DETs

77

41

9

  

# DETs

10

47

79

  

Urinary PC

 ~ − 0.67

− 0.67 to 0.18

0.18 ~ 

  

Biosensor PC

− 0.24 (− 0.56 to 0.08)

0.02 (− 0.29 to 0.32)

0.29 (− 0.03 to 0.61)

0.27 (0.03 to 0.50)

0.025

Delta Ct

     

 CCL2

− 8.66 (− 9.17 to − 8.14)

− 8.35 (− 8.85 to − 7.86)

− 8.25 (− 8.76 to − 7.75)

0.20 (− 0.17 to 0.57)

0.289

 CCL5

− 1.65 (− 1.93 to − 1.36)

− 1.50 (− 1.78 to − 1.23)

− 1.13 (− 1.41 to − 0.85)

0.26 (0.06 to 0.47)

0.013

 CXCL8

− 8.99 (− 9.68 to − 8.31)

− 9.29 (− 9.95 to − 8.63)

− 9.13 (− 9.82 to − 8.44)

− 0.07 (− 0.57 to 0.43)

0.782

 CXCL12

− 3.23 (− 3.56 to − 2.90)

− 3.01 (− 3.33 to − 2.70)

− 2.87 (− 3.19 to − 2.55)

0.18 (− 0.06 to 0.42)

0.135

 ICAM

− 5.61 (− 5.98 to − 5.25)

− 5.30 (− 5.66 to − 4.95)

− 5.16 (− 5.52 to − 4.80)

0.23 (− 0.04 to 0.49)

0.095

 SELP

0.10 (− 0.16 to 0.36)

0.19 (− 0.06 to 0.44)

0.47 (0.21 to 0.72)

0.19 (− 0.001 to 0.37)

0.052

 VCAM

− 3.20 (− 3.66 to − 2.75)

− 2.75 (− 3.19 to − 2.31)

− 2.48 (− 2.93 to − 2.02)

0.36 (0.03 to 0.69)

0.032

Relative quantification

     

 CCL2

403.10 (282.28 to 575.23)

326.97 (231.84 to 461.12)

305.28 (214.67 to 434.14)

0.87 (0.67 to 1.13)

0.289

 CCL5

3.13 (2.57 to 3.82)

2.83 (2.34 to 3.43)

2.18 (1.80 to 2.66)

0.83 (0.72 to 0.96)

0.013

 CXCL8

509.52 (316.93 to 818.59)

626.86 (397.0 to 990.49)

561.83 (348.25 to 905.77)

1.05 (0.74 to 1.48)

0.782

 CXCL12

9.37 (7.47 to 11.75)

8.08 (6.49 to 10.06)

7.31 (5.84 to 9.14)

0.88 (0.75 to 1.04)

0.135

 ICAM

48.87 (37.92 to 62.94)

39.42 (30.87 to 50.39)

35.75 (27.78 to 46.01)

0.86 (0.71 to 1.03)

0.095

 SELP

0.93 (0.78 to 1.12)

0.88 (0.74 to 1.04)

0.72 (0.61 to 0.86)

0.88 (0.77 to 1.00)

0.052

 VCAM

9.22 (6.70 to 12.67)

6.72 (4.95 to 9.15)

5.57 (4.07 to 7.63)

0.78 (0.62 to 0.98)

0.032

  1. Urinary PC was the first PC generated based on six urinary PAH metabolites and correlated strongly with diesel exhaust exposure (r > 0.64). Biosensor PC was extracted based on delta Ct of 7 ex vivo biosensor genes and explained 39% of total variance
  2. aGeneralized linear model was used to assess the association between internal dose of diesel exhaust exposure and ex vivo biosensor PC in 124 DETs and 123 non-DETs with adjustment for age, obesity, internal dose of cigarette smoking, and passage of cells. All significant associations did not vary by smoking status
Back to article page

Particle and Fibre Toxicology

ISSN: 1743-8977

Contact us