Fig. 5
The role of ROS production and oxidative stress in Programmed cell death induced by GO, MCLR and GO-MCLR. HaCaT cells and L02 cells at logarithmic growth stage were inoculated in 12-well plates at a density of 1 × 105/mL. a Intracellular ROS production identified by DCFH-DA in HaCaT and L02 cells treated with GO (5 µg/mL and 50 µg/mL), MCLR (0.06 µg/mL and 0.6 µg/mL) and GO-MCLR complex (5 µg/mL + 0.06 µg/mL, 50 µg/mL + 0.6 µg/mL) for 24 h using fluorescence microscopy. Excitation wavelength of 488 nm and emission wavelength of 525 nm for the incidence of intracellular ROS. The activities of SOD (b), MDA (c) and GSH (d) in treated cells were detected by corresponding kits. *P < 0.05 versus Control, #P < 0.05 versus GO group