Fig. 7

Identification of specific injury induced by MCLR before and after GO adsorption. HaCaT and L02 cells were co-incubated with GO (5 µg/mL and 50 µg/mL), MCLR (0.06 µg/mL and 0.6 µg/mL) and GO-MCLR complex (5 µg/mL + 0.06 µg/mL, 50 µg/mL + 0.6 µg/mL) suspensions for 24 h. a After 24 h of treatment, the cells culture medium was collected and analyzed for PP2A levels. b Cells cytoskeleton damage was determined via FITC-Phallodin staining. After 24 h of exposure, the cells were fixed with 4% paraformaldehyde dissolved in PBS for 10 min and treated at room temperature for 5 min with 0.5%Triton X-100 dissolved in PBS. 200 µL FITC-Phallodin staining solution was added and incubated for 30 min at room temperature without light. 100 µL anti-fluorescence quenching agent containing DAPI was added, and the staining results were imaged under laser confocal microscopy. The FITC (Ex/Em = 492/518 nm) and DAPI (Ex/Em = 364/454 nm) excitation/emission filters were selected