Fig. 9
SOD1 redistribution in CuO NP-exposed cells. SOD1 localization in RAW264.7 cells was determined by confocal microscopy. SOD1 was visualized using a specific antibody and an Alexa Fluor® 488-conjugated secondary antibody (green). Mitochondria were detected using MitoTracker™ Red and cell nuclei were counterstained using DAPI (blue). a Control; b CuO NPs (10 µg/mL); c CuO NPs (25 µg/mL); d CuCl2 (50 µg/mL). The cells were exposed for 6 h