Fig. 15
Effect of pretreatment with clodronate liposome and PMN-neutralizing antibody on the pulmonary acute toxicity of CWAAP-A. Male rats were given a single intratracheal dose of clodronate liposomes (1 ml/kg), or a single intravenous injection of PMN antibodies (0.5 ml/kg). They were then exposed to 10 mg/m3 of CWAAP-A for 6 h, and their plasma and BALF were collected the next day. The number of white blood cells (WBC) (a), monocytes (b) and neutrophils (c) in plasma, and the number of total cells (d), alveolar macrophage (AM) (e) and neutrophils (f) in the BALF were measured by an automated hematology analyzer. The plasma concentration of SP-D was determined by an enzyme immunoassay (g). Tukey’s multiple comparison test: *p < 0.05, **p < 0.01, and ***p < 0.001, pairs indicated