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Table 1 The number of rats in each group for each test

From: Aluminium oxide nanoparticles compromise spatial memory performance and proBDNF-mediated neuronal function in the hippocampus of rats

Test

Group

Control

AluminaNP-10

AluminaNP-20

AluminaNP 10-TATPep5

TATPep5

Ro25

AluminaNP-10-Int

AluminaNP-10-Exp

Behavioral tests (Fig. 1)

 MWM

  Acquisition phase

16

19

17

–

–

–

–

–

  STM test

5

5

5

–

–

–

–

–

  LTM test

6

7

6

–

–

–

–

–

  Retrieval-LTM

5

7

6

–

–

–

–

–

Open field/ Level press

  –

6

6

6

–

–

–

–

–

Western-blot tests

  mBDNF/CREB /proBDNF/RhoA

5

7

7

–

–

–

–

–

MWM (Fig. 2)

  LTM test

5

5

–

8

5

–

–

–

Slice recordings

  I/O and LTD

5

6

–

6

4

3

6

4

  EPSC amplitude /frequency

6

6

–

7

5

–

–

–

Slice ROS

  Superoxide/hydroxyl free radical

5

5

–

5

4

–

–

–

In vivo recordings

  IN/PN neurons

5

7

–

8

5

–

–

–

  1. All experiments were performed on separate groups. Following acquisition training (total n = 52), rats from independent subgroups were tested short-term memory (STM, n = 15), long-term memory (LTM, n = 19) and the retrieval phase of LTM (n = 18). The LTM test in the water-maze task was conducted from subgroups without infusions during the acquisition training. The samples used for detecting levels of mBDNF/CREB/proBDNF/RhoA were obtained from the same subgroup. The levels of ROS were detected from the slices without simulations or EPSC recordings