Fig. 3

scRNA-seq of mouse whole lung cells in response to sub-chronic PM exposure. A Schematic diagram of single-cell samples preparation. B UMAP plot of whole lung cells in control and PM exposed groups. Cells from whole lungs of control or exposed group are indicated by red or turquoise color. C Thirteen clusters with 11 cell types identified on the UMAP plot. D The dot plot represents the expression level of the representative marker genes (dot color shades) and the percentage of cells expressing marker genes (dot size). E The heatmap illustrates the number of whole lung cells in control and PM exposure groups. F Expression levels of proinflammatory and profibrotic genes including S100a8, S100a9, Fn1, Mmp8, Col1a1, and Col3a1 on UMAP plots split by groups of different exposure status (N = 3). AT, alveolar epithelial cells; Con, control group; Exp, PM exposure group; UMAP, Uniform Manifold Approximation and Projection