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Fig. 5 | Particle and Fibre Toxicology

Fig. 5

From: Single-cell transcriptomics reveals immune dysregulation mediated by IL-17A in initiation of chronic lung injuries upon real-ambient particulate matter exposure

Fig. 5

IL-17A deficiency attenuates PM-induced chronic lung injuries. A The inflammatory status of representative H&E-stained lung sections in 8-week and 16-week PM exposure groups and their corresponding control groups in WT and IL-17A−/− mice. Yellow bold arrows indicate the interstitial neutrophils infiltration. B ALI scores assessed based on the pathological changes shown in A (N = 8). C Collagen content (%) in lung tissue was calculated as the ratio of labeled blue areas to total area of lung section (%/μm2 total area) upon Masson's trichrome staining (N = 8). D Representative Masson's trichrome-stained lung sections indicate the profibrotic changes including collagen deposition, knot-like formation, and thickened alveolar septum in 16-week PM exposure group from WT and IL-17A−/− mice (N = 8). E Ashcroft scores were calculated in the WT and IL-17A−/− mice in control and PM exposed groups following 8-week and 16-week exposure (N = 8). F The levels of TGF-β in lung tissue from WT and IL-17A−/− mice in control and PM exposure groups following 8-week and 16-week exposure (N = 3). Photographs were obtained through scanning by TissueFAXS analysis system with the 200X magnification. Scale bar = 50 μm. The results are shown as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001 PM-exposed mice compared with the control mice (WT−-PM vs WT-Con). #P < 0.05 compared with the WT PM-exposed mice (IL-17A−/−-PM vs WT-PM); ###P < 0.001 IL-17A−/−-PM vs WT-PM. Con: control group; Exp: PM exposure group; ALI: acute lung injury

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