Fig. 6

Identification of immunosuppressive cells in neutrophil and monocyte-derived cell subset following sub-chronic PM exposure. A Circos plot is depicted for significant alterations (PM exposure group vs control group) in cellular interactions among neutrophils, monocyte-derived cells (displayed as monocytes), fibroblasts, alveolar macrophages, and T cells. B Sub-clusters including monocytes (cluster 0 and 1), dendritic cells (cluster 2), and interstitial macrophages (cluster 3) are identified from the UMAP plot of monocyte-derived cell subset. C Pseudotime trajectory analysis in the left panel indicates the differentiation state of the monocyte-derived cells as shown in red (monocytes), green (interstitial macrophages), or blue (dendritic cells). The other one in the right panel was colored from dark blue to light blue, indicating pseudotime scores from the lowest to the highest in monocyte-derived cells. D Each dot represents the expression of Wfdc17 in the UMAP plots from the monocyte-derived cell subset, colored from grey to red, indicating the low to high expression. Cells from control and PM exposed group were shown in the separate column. E Sub-clusters are identified in neutrophil subset on UMAP plot. F The results of pseudotime trajectory analysis were split by exposure status (left panel) and colored based on pseudotime scores (right panel) in neutrophil subset, from dark blue to light blue, indicating from the lowest to the highest score. G The expression of Wfdc17, Arg2, Il1b, and Ifitm1 was shown in UMAP plots from control and PM exposed groups, which was displayed by the colors from grey to red. Cells from control and PM exposed groups were shown in the separate columns. Con: control group; Exp: PM exposure group; UMAP: Uniform Manifold Approximation and Projection