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Fig. 4 | Particle and Fibre Toxicology

Fig. 4

From: Road tunnel-derived coarse, fine and ultrafine particulate matter: physical and chemical characterization and pro-inflammatory responses in human bronchial epithelial cells

Fig. 4

Cytokine responses in HBEC3-KT cells after exposure to PM samples from two road tunnels. The cells were exposed to coarse, fine and ultrafine PM samples from the Marienborg and Hell road tunnels during dry and humid road surface conditions. The cells were exposed to 100 µg/mL (10.4 µg/cm2) PM samples for 20 h. The cytokine release was analysed by ELISA. A CXCL8; B IL-6; C IL-1α; D IL-1β. The data represent the mean ± SEM of 5 experiments. Statistical analysis was performed by two-way ANOVA (repeated measurements) with Tukey’s multiple comparisons test. *Significantly different from control, # significantly different between groups, p < 0.05

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