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Fig. 1 | Particle and Fibre Toxicology

Fig. 1

From: Titanium dioxide and carbon black nanoparticles disrupt neuronal homeostasis via excessive activation of cellular prion protein signaling

Fig. 1

TiO2 and CB nanoparticles interact with PrPC. a Schematic of the experimental procedure to assess the interaction between full-length recombinant mouse PrPC (recPrPC, 2 µM) and TiO2- or CB-NPs (0–80 µg ml−1) exploiting the intrinsic fluorescence of PrPC or by western blotting (WB). b Fluorescence titration curves showing the binding of TiO2- or CB-NPs to full-length mouse recPrPC. The quantity of recPrPC bound with nanoparticles was deduced by subtracting the fluorescence level of titrated free PrPC to the fluorescence level of total PrPC measured in the absence of nanoparticles. Fitting hyperbolic curves were calculated with the help of the Kaleidagraph Software (Abelbeck Software). c Representative Western-blot and quantification histogram showing decrease of free recPrPC amount in the supernatant of the centrifuged reaction medium between recPrPC and TiO2- or CB-NPs. The experiments were performed three times in triplicates. Values are means ± SEM. *denotes p < 0.05, ***p < 0.001, ****p < 0.0001 versus recPrPC incubated without nanoparticles

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