Fig. 2
PrPC facilitates nanoparticle interaction with plasma membrane of 1C11 precursors and 1C115−HT neuronal cells. a, b Transmission Electron Microscopy experiments showing large aggregates of TiO2- and CB-NPs (white arrows) within 1C11 precursor cells and 1C115−HT neuronal cells a and small aggregates of TiO2- and CB-NPs (red arrows) at the plasma membrane of 1C11 and 1C115−HT cells b after 24 h exposure to 10 µg cm−2 nanoparticles. Scale bar = 2 µm in a. Scale bar = 0.5 µm in b. c Light scattering-based FACS analysis of interacting TiO2-NPs with the surface of 1C11 and PrPnull-1C11 cells exposed up to 10 µg cm−2 nanoparticles. (Left) Representative bright-field images of 1C11 and PrPnull-1C11 cells exposed to 5 µg cm−2 TiO2-NPs merged with the light scattering signal of TiO2-NPs (pink signal) adsorbed to the plasma membrane after internal masking of cells (see Methods). (Right) Quantification histogram of TiO2-NPs present at the surface of 1C11 and PrPnull-1C11 cells. Scale bar = 5 µm. The experiments were performed three times in triplicates. Values are means ± SEM. *denotes p < 0.05 versus 1C11 cells exposed to nanoparticles