Fig. 3
TiO2- and CB-NPs specifically interact with full-length PrPC in the 1C11 neuronal cell line. a Schematic of the experimental procedure to assess the interaction of TiO2- and CB-NPs with PrPC expressed at the plasma membrane of 1C11 precursor cells and their serotonergic 1C115−HT neuronal progenies by western blotting. b Quantification histogram deduced from Western-blot experiments showing the amounts of full-length (FL) PrPC (left) and C1 fragment (right) in the supernatant (S fraction) obtained after lysis of 1C11 cells exposed to increasing concentrations of TiO2- or CB-NPs (0 to 10 µg cm−2) for 15 min and centrifugation of the lysates. c, d Representative Western-blots and quantification histograms showing time-variation in the level of FL PrPC and C1 fragment in the S and P fractions derived from 1C11 c and 1C115−HT d cells exposed to TiO2- or CB-NPs (1 µg cm−2). α-tubulin was used for normalization of PrPC level in the S fraction. The experiments were performed three times in triplicates. Values are means ± SEM. *denotes p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 versus unexposed cells