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Fig. 4 | Particle and Fibre Toxicology

Fig. 4

From: Titanium dioxide and carbon black nanoparticles disrupt neuronal homeostasis via excessive activation of cellular prion protein signaling

Fig. 4

TiO2 and CB nanoparticle interaction with PrPC promotes NADPH oxidase-dependent ROS production. a Kinetics of ROS production induced on exposure to TiO2- and CB-NPs (1 µg cm−2) in 1C11 and 1C115−HT neuronal cells. b Involvement of NADPH oxidase in NP-induced ROS production (1 µg cm−2 for 2 h) using the NADPH oxidase inhibitor apocynin (500 µM). c Quantification histogram showing that siRNA-based PrPC silencing (siPrP) abrogates ROS production induced by TiO2- or CB-NPs (1 µg cm−2 for 2 h) in 1C11 and 1C115−HT cells. d GSH level keeps constant in 1C11 and 1C115−HT neuronal cells exposed to TiO2- or CB-NPs (1 µg cm−2) up to 24 h. The experiments were performed three times in triplicates. Values are means ± SEM. *denotes p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 versus unexposed cells

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