Fig. 6

TiO₂ and CB nanoparticle interaction with PrP^C promotes TACE internalization in a PDK1-dependent manner at the root of TNFR1 overexposure at the cell surface. a TACE immunostaining at the plasma membrane of 1C11 cells exposed to TiO₂- or CB-NPs (1 µg cm⁻²) for 1 h in the presence or not of a siRNA toward PrP^C (siPrP) or the PDK1 inhibitor, BX912 (1 µM) and related quantification histogram. Cell permeabilization with saponin (0.05%) shows TACE internalization in 1C11 cells exposed to nanoparticles. Scale bar = 10 µm. b, c TACE expression level as assessed by RT-qPCR b and Western-blotting c in 1C11 cells exposed to TiO₂- or CB-NPs (1 µg cm⁻²) for 1 h. d PDK1 phosphorylation status at Ser241 (p-PDK1) was assessed by Western-blotting in 1C11 cells exposed to TiO₂- or CB-NPs (1 µg cm⁻²) for 1 h. e TNFR1 immunostaining at the plasma membrane of 1C11 cells exposed for 1 h to TiO₂- or CB-NPs (1 µg cm⁻²) in the presence or not of a siRNA toward PrP^C (siPrP) or the PDK1 inhibitor, BX912 (1 µM) and related quantification histogram. Scale bar = 10 µm. The experiments were performed three times in triplicates. Values are means ± SEM. *denotes p < 0.05, **p < 0.01, ***p < 0.001 versus unexposed cells