Fig. 1

Mice were kept in exposure chambers for six months. Graphical illustrations A) Mouse in filtered air chamber. B) Mouse in dirty air chamber. C) Movement of ultrafine PM particles into the respiratory track and accumulation in lungs and heart. D) Normalized fluorescence spectra of ultrafine PM in PBS (green) and auto-fluorescence of tissues (red). E) Typical lifetime decay curves of ultrafine PM in tissues (green) and auto-fluorescence of tissues (red). F) Auto-fluorescent image of lung tissue from filtered air. G) Ultrafine PM particles from filtered air. H) Fluorescent deposition pattern of ultrafine PM particles in lung tissue from filtered air. I) Auto-fluorescent image of lung tissue from dirty air. J) Ultrafine PM particles from dirty air. K) Fluorescent deposition pattern of ultrafine PM particles in lung tissue from dirty air. L) Auto-fluorescent image of heart tissue from filtered air. M) Green dots are the ultrafine PM particles. N) Fluorescent deposition pattern of ultrafine PM particles in heart tissue from filtered air. O) Auto-fluorescent image of heart tissue from dirty air. P) Green dots are the ultrafine PM particles. Q) Fluorescent deposition pattern of ultrafine PM particles in heart tissue from dirty air. Scale bar: 20 µm. The resolution of FLIM images is approximately 250 nm. R) The estimated particle density in lung and heart of mice