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Fig. 2 | Particle and Fibre Toxicology

Fig. 2

From: Label-free detection and quantification of ultrafine particulate matter in lung and heart of mouse and evaluation of tissue injury

Fig. 2

Field emission scanning electron microscopy (FE-SEM) of lung tissues. A) Lung tissues from filtered air showed no signs of abnormality, whereas B, C lung tissue sections from dirty air exposure group showed ultrafine PM particles, D–F macrophages. High magnification FE-SEM image of the macrophages showed cell surface, knob like microvilli, and filopodia that extended outwards from periphery of the cells (F). Lung tissue sections from dirty air exposure group showed amyloid deposits (G–I), and fibrosis (J–L) Congo red staining, M lung tissues from filtered air (control) showed no signs of abnormality, whereas N lung tissue from the dirty air showed amyloid deposition. Immunohistochemistry with amyloid marker Aβ antibody (1:500), O from lung tissue sections from filtered air (control) group showed no signs of abnormality, whereas P lung tissue from dirty air showed immunoreactive areas with dark brown amyloid deposits. Immunohistochemistry with macrophage marker IBA-1 antibody (1:100), Q lung tissues from filtered air showed no signs of abnormality, whereas R lung tissue from dirty air showed macrophages. Magnification (A, B, D, G, J, and K) 20 k, scale bar: 2 µm. Magnification (E, H, I, and L) 50 k, Scale bar: 1 µm. Magnification (C and F) 100 k, Scale bar: 500 nm. Magnification (M and N) 20X, Scale bar: 500 µm. Magnification (O, P, Q, and R) 40X, Scale bar 500 µm

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