Fig. 3From: Label-free detection and quantification of ultrafine particulate matter in lung and heart of mouse and evaluation of tissue injuryField emission scanning electron microscopy (FE-SEM) of heart tissues. A Heart tissues from filtered air (control) group showed no signs of abnormality, whereas B, C heart tissue from dirty air exposure group showed ultrafine PM particles, D, E amyloid, and F macrophages. Histopathological evaluation by Congo red staining, G heart tissues from filtered air (control) group showed no signs of abnormality, whereas H, I heart tissue from the dirty air showed amyloid deposition. Immunohistochemistry with amyloid marker Aβ antibody (1:500), J from heart of filtered air (control) group showed no signs of abnormality, whereas) heart tissue from dirty air showed immunoreactive areas with dark brown amyloid deposition (K and L). Immunohistochemistry with macrophage marker IBA-1 antibody (1:100), M heart tissues from filtered air (control) group showed no signs of abnormality, whereas (N and O) heart tissue from dirty air showed macrophages. Magnification (A to D) 10 k, scale bar: 5 μm. Magnification (E) 20 k, scale bar 2 μm. Magnification (F) 100 k, scale bar: 500 nm. Magnification (G, H, I, J, L, M, N, and O) 40X, Scale bar: 500 µm. Magnification (K) 20X, Scale bar: 500 µmBack to article page