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Fig. 3 | Particle and Fibre Toxicology

Fig. 3

From: Macrophage-mediated tissue response evoked by subchronic inhalation of lead oxide nanoparticles is associated with the alteration of phospholipases C and cholesterol transporters

Fig. 3

Lung after 11-week PbO NP inhalation. A, D Lungs in control animals without alternations. B, C, E Exposure to PbO NPs caused remodeling of lung tissue in alveolar areas (a). There are peribronchiolar (b) or perivascular (bv) inflammatory infiltrates of leukocytes (il) after PbO NPs inhalation. Arrow shows hemosiderin. F Evaluation of histopathological changes after 11 weeks of lead oxide nanoparticle inhalation according to the Table S1. The graphs values denote average ± SD; ***p < 0.001 by unpaired t-test. G–I Amount of collagen fibres (green) is not changed after inhalation of PbO NPs. Collagen fibers are around blood vessels (bv) and bronchioles (b). There are not any collagen fibers in alveolar areas despite serious remodeling. J–L MPO detection in lung tissue. Arrows display myeloperoxidase-positive cells—neutrophils. M–O Mastocytes (arrows) in lungs, insert displays the number of mastocytes per slide. Scale bar in all panels = 100 µm. P–R The ultrastructural morphology of the lung tissue with inflammatory features. P Terminal bronchiole lined with secretory club (cl), basal (ba) and ciliated (ci) cells; macrophage (ma) with cholesterol crystals inside (arrow) the lumen. Q Numerous macrophages (ma), neutrophils (ne) and abundant cell debris (de) in lung alveoli. R Clump of plasma cells (pl) around vessel in alveolar septum. S, T, T′ Endosomes with PbO nanoparticles (nps) in pneumocyte type I. Scale bars are displayed individually for each picture

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Particle and Fibre Toxicology

ISSN: 1743-8977

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